P2 laboratory

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Manager : Dr Stéphane GUYOT

For handling class 2 pathogenic bacteria (Listeria, Salmonella...)  

 

Access and use of the P2 laboratory are governed by internal regulations validated by the AgroSup Dijon Health & Safety, Working Conditions & Prevention Dept., the University of Burgundy Health and Safety Dept., the occupational health dept. of the Agricultural Social Security Fund of Burgundy (MSA), and the preventive medicine dept. (for students and staff).

A research project is currently underway:

  • 2015-2018ANR-funded BlacHP (Lactic Bacteria combined with High Pressure for more sustainable stabilisation of refrigerated meat products) [Project Leader: PAM Joint Research Centre, Prof. Jean-Marie PERRIER-CORNET PhD supervisor (PMB team), Dr Hélène SIMONIN joint supervisor (PMB team)]. Chloé MODUGNO, a doctoral student at the PAM Joint Research Centre, is working on this project.

Previous research projects:

  • 2013-2016 ANR-funded EcoSec (Surface decontamination in workshops in the food industry by controlling the relative humidity to reduce biocide consumption; the study was conducted on a model microorganism: Listeria monocytogenes) [Project leader: ANSES; partner: PAM Joint Research Centre (Prof. Laurent BENEY PhD supervisor (PMB team) and lead scientist for the EcoSec project, Dr Stéphane GUYOT joint supervisor (PMB team), Dr Cosette GRANDVALET joint supervisor (VALMIS team))]. Fiona ZOZ, a doctoral student at the PAM Joint Research Centre, is working on this project.

  • 2013-2016 CIFRE agreement PhD in collaboration with Novolyze (Karim Kinouche): Destruction of pathogenic microorganisms using a thermal process. Emilie LANG, a doctoral student, is assigned to this project. PMB team members: Prof. Patrick GERVAIS (PhD supervisor), Prof. Jean-Marie PERRIER-CORNET (joint PhD supervisor), Dr Stéphane GUYOT (joint PhD supervisor).

Contact

P2 laboratory manager:

Phone:

List of equipment

3 Microbiological safety cabinets
1 Benchtop flow cytometer with a microplate feeder
1 Spectrophotometer
1 Thermocycler
1 Fridge
1 Freezer
2 Ovens
1 Centrifuge (temperature regulation possible)
1 Anaerobic chamber
1 Aspiration system equipped with 0.2 µm filters

Laboratory for the culture and preservation of obligate anaerobic microorganisms

Manager

Importance of anaerobic cultures

The intestinal microbiota comprises 100 thousand billion bacterial cells, ten times more than human cells (1). Most of these bacteria can be found in the colon where the environmental conditions are completely anaerobic, and so the majority of bacteria comprising our microbiota are obligate anaerobes (2), which require complex nutritious and gaseous environmental conditions for their growth.

Numerous studies have demonstrated the importance of understanding better these anaerobic bacteria and their impact on the health of the host. Indeed, a low abundance of these bacteria in the intestinal microbiota has already been correlated with the development of chronic intestinal diseases (3,4,5). The PAM research centre has taken up the challenge of developing more sensitive biotechnological tools for the culture, stabilisation, and preservation of these bacteria within the framework of several collaborative projects involving academic and industrial partners.

The importance of anaerobic cultures does not stop with the human microbiota; this issue is frequently encountered during the production of biofuel using bacteria or when studying ecological niches characterised by the absence of oxygen such as the seabed.

An anaerobic laboratory at the cutting edge of biotechnology

The PAM research centre has the human and material resources to study microorganisms with extreme oxygen sensitivity (EOS) and therefore difficult to cultivate and preserve. The centre also addresses issues relating to the impact of modifying the gaseous atmosphere on the properties of microorganisms and their reactions to stress (6).

Promising results within the framework of an FUI – government funded project on the production of an obligate anaerobic EOS bacterium have already been obtained with regard to the large-scale production of biomass and in terms of optimising the viability of bacteria after a long period of preservation.

Oral communication at the Congress on Useful Microbial Flora held on 7 October 2004: The problem case of obligate anaerobic bacteria

 

List of equipment

Equipment: 

  • 1 Sheldon Bactron II anaerobic chamber

  • 1 Freeze dryer (18 litres)

  • 1 Tri-gas oven*

  • 2 fermenters (1 litre)*

  • 3 fermenters (5 litres)*

  • 1 MSC

  • 1 Glove box*

Material: 

Liquid cultures:

  • Hungate tubes

  • Schott* systems (up to 500 ml)

Plate cultures:

  • Gas-Pak* jars

  • Oven integrated in the Sheldon Bactron II anaerobic chamber

*Anaerobe Ready